AarI	CACCTGCNNNN^	ISO	
AarI+	^NNNNNNNNGCAGGTG	ISO	
Acc36I	ACCTGCNNNN^	ISO	BfuAI	BspMI	BveI	
Acc36I+	^NNNNNNNNGCAGGT	ISO	BfuAI	BspMI	BveI	
AccBSI	CCG^CTC	ISO	BsrBI	MbiI	
AccBSI+	GAG^CGG	ISO	BsrBI	MbiI	
AciI	C^CGC	ISO	BspACI	SsiI	
AciI+	G^CGG	ISO	BspACI	SsiI	
AclWI	GGATCNNNN^	ISO	AlwI	BspPI	
AclWI+	^NNNNNGATCC	ISO	AlwI	BspPI	
AcuI	CTGAAGNNNNNNNNNNNNNNNN^	ISO	Eco57I	
AcuI+	^NNNNNNNNNNNNNNCTTCAG	ISO	Eco57I	
AjiI	CAC^GTC	ISO	BmgBI	BtrI	
AjiI+	GAC^GTG	ISO	BmgBI	BtrI	
Alw26I	GTCTCN^	ISO	BcoDI	BsmAI	BstMAI	
Alw26I+	^NNNNNGAGAC	ISO	BcoDI	BsmAI	BstMAI	
AsuHPI	GGTGANNNNNNNN^	ISO	HphI	
AsuHPI+	^NNNNNNNTCACC	ISO	HphI	
BauI	C^ACGAG	ISO	BssSI	Bst2BI	
BauI+	C^TCGTG	ISO	BssSI	Bst2BI	
BbsI	GAAGACNN^	ISO	BpiI	BpuAI	BstV2I	
BbsI+	^NNNNNNGTCTTC	ISO	BpiI	BpuAI	BstV2I	
BbvCI	CC^TCAGC	ISO	
BbvCI+	GC^TGAGG	ISO	
BbvI	GCAGCNNNNNNNN^	ISO	BseXI	BstV1I	Lsp1109I	
BbvI+	^NNNNNNNNNNNNGCTGC	ISO	BseXI	BstV1I	Lsp1109I	
BccI	CCATCNNNN^	ISO	
BccI+	^NNNNNGATGG	ISO	
BceAI	ACGGCNNNNNNNNNNNN^	ISO	
BceAI+	^NNNNNNNNNNNNNNGCCGT	ISO	
BciVI	GTATCCNNNNNN^	ISO	BfuI	BsuI	
BciVI+	^NNNNNGGATAC	ISO	BfuI	BsuI	
BfiI	ACTGGGNNNNN^	ISO	BmrI	BmuI	
BfiI+	^NNNNCCCAGT	ISO	BmrI	BmuI	
BmsI	GCATCNNNNN^	ISO	LweI	SfaNI	
BmsI+	^NNNNNNNNNGATGC	ISO	LweI	SfaNI	
BpmI	CTGGAGNNNNNNNNNNNNNNNN^	ISO	GsuI	
BpmI+	^NNNNNNNNNNNNNNCTCCAG	ISO	GsuI	
Bpu10I	CC^TNAGC	ISO	
Bpu10I+	GC^TNAGG	ISO	
BpuEI	CTTGAGNNNNNNNNNNNNNNNN^	ISO	
BpuEI+	^NNNNNNNNNNNNNNCTCAAG	ISO	
BsaI	GGTCTCN^	ISO	Bso31I	Eco31I	
BsaI+	^NNNNNGAGACC	ISO	Bso31I	Eco31I	
Bse1I	ACTGGN^	ISO	BseNI	BsrI	BsrSI	
Bse1I+	C^CAGT	ISO	BseNI	BsrI	BsrSI	
Bse3DI	GCAATGNN^	ISO	BseMI	BsrDI	
Bse3DI+	CATTGC^	ISO	BseMI	BsrDI	
BseGI	GGATGNN^	ISO	BstF5I	BtsCI	
BseGI+	CATCC^	ISO	BstF5I	BtsCI	
BseMII	CTCAGNNNNNNNNNN^	ISO	
BseMII+	^NNNNNNNNCTGAG	ISO	
BseRI	GAGGAGNNNNNNNNNN^	ISO	
BseRI+	^NNNNNNNNCTCCTC	ISO	
BseYI	C^CCAGC	ISO	
BseYI+	G^CTGGG	ISO	
BsgI	GTGCAGNNNNNNNNNNNNNNNN^	ISO	
BsgI+	^NNNNNNNNNNNNNNCTGCAC	ISO	
BslFI	GGGACNNNNNNNNNN^	ISO	BsmFI	FaqI	
BslFI+	^NNNNNNNNNNNNNNGTCCC	ISO	BsmFI	FaqI	
BsmBI	CGTCTCN^	ISO	Esp3I	
BsmBI+	^NNNNNGAGACG	ISO	Esp3I	
BsmI	GAATGCN^	ISO	Mva1269I	PctI	
BsmI+	G^CATTC	ISO	Mva1269I	PctI	
BspCNI	CTCAGNNNNNNNNN^	ISO	
BspCNI+	^NNNNNNNCTGAG	ISO	
BspQI	GCTCTTCN^	ISO	LguI	PciSI	SapI	
BspQI+	^NNNNGAAGAGC	ISO	LguI	PciSI	SapI	
Bst6I	CTCTTCN^	ISO	Eam1104I	EarI	
Bst6I+	^NNNNGAAGAG	ISO	Eam1104I	EarI	
BtgZI	GCGATGNNNNNNNNNN^	ISO	
BtgZI+	^NNNNNNNNNNNNNNCATCGC	ISO	
BtsI	GCAGTGNN^	ISO	
BtsI+	CACTGC^	ISO	
BtsIMutI	CAGTGNN^	ISO	
BtsIMutI+	CACTG^	ISO	
CseI	GACGCNNNNN^	ISO	HgaI	
CseI+	^NNNNNNNNNNGCGTC	ISO	HgaI	
EciI	GGCGGANNNNNNNNNNN^	ISO	
EciI+	^NNNNNNNNNTCCGCC	ISO	
Eco57MI	CTGRAGNNNNNNNNNNNNNNNN^	ISO	
Eco57MI+	^NNNNNNNNNNNNNNCTYCAG	ISO	
FauI	CCCGCNNNN^	ISO	SmuI	
FauI+	^NNNNNNGCGGG	ISO	SmuI	
FokI	GGATGNNNNNNNNN^	ISO	
FokI+	^NNNNNNNNNNNNNCATCC	ISO	
FspEI	CCNNNNNNNNNNNN^	ISO	
FspEI+	^NNNNNNNNNNNNNNNNGG	ISO	
GsaI	CCCAG^C	ISO	
GsaI+	GCTGG^G	ISO	
HpyAV	CCTTCNNNNNN^	ISO	
HpyAV+	^NNNNNGAAGG	ISO	
LpnPI	CCDGNNNNNNNNNN^	ISO	
LpnPI+	^NNNNNNNNNNNNNNCHGG	ISO	
MboII	GAAGANNNNNNNN^	ISO	
MboII+	^NNNNNNNTCTTC	ISO	
MlyI	GAGTCNNNNN^	ISO	SchI	
MlyI+	^NNNNNGACTC	ISO	SchI	
MmeI	TCCRACNNNNNNNNNNNNNNNNNNNN^	ISO	
MmeI+	^NNNNNNNNNNNNNNNNNNGTYGGA	ISO	
MnlI	CCTCNNNNNNN^	ISO	
MnlI+	^NNNNNNGAGG	ISO	
MspJI	CNNRNNNNNNNNN^	ISO	
MspJI+	^NNNNNNNNNNNNNYNNG	ISO	
NmeAIII	GCCGAGNNNNNNNNNNNNNNNNNNNNN^	ISO	
NmeAIII+	^NNNNNNNNNNNNNNNNNNNCTCGGC	ISO	
PleI	GAGTCNNNN^	ISO	PpsI	
PleI+	^NNNNNGACTC	ISO	PpsI	
TaqII	GACCGANNNNNNNNNNN^	ISO	
TaqII+	^NNNNNNNNNTCGGTC	ISO	
TsoI	TARCCANNNNNNNNNNN^	ISO	
TsoI+	^NNNNNNNNNTGGYTA	ISO	
TspDTI	ATGAANNNNNNNNNNN^	ISO	
TspDTI+	^NNNNNNNNNTTCAT	ISO	
TspGWI	ACGGANNNNNNNNNNN^	ISO	
TspGWI+	^NNNNNNNNNTCCGT	ISO